31 Jul Carrier erythrocytes, resealed erythrocytes loaded by a drug or other therapeutic agents, have been exploited extensively in recent years for. Carrier erythrocytes, resealed erythrocytes loaded by a drug or other therapeutic agents, have been exploited extensively in recent years for both temporally and. Current review highlights isolation, drug loading methods, Evaluation methods and applications of resealed erythrocytes for drug delivery. Keywords: Resealed .

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Numerous application have been proposed for the use of resealed erythrocytes as carrier for drugsenzyme replacement therapy etc.

Also known as electroporation, the method is based on the observation that electrical shock brings about irreversible changes in an erythrocyte membrane. Acta Physiol Acad Sci Hung ; 6: The medium is agitated slowly for 2 hr.

The major drawbacks of this method include low entrapment efficiency and a considerable loss of hemoglobin and other cell components. The entrapment of material occurs by endocytosis. Antineoplastic drugs such as methotrexate, bleomycin, asparginase, and adriamycin 74 have been successfully delivered by erythrocytes.

Erythrocytes have an exceptional capability for reversible shape changes with or without accompanying volume change and for reversible deformation under stress. The ability of resealed erythrocyte to deliver drug to the liver or spleen can be viewed as a disadvantage is that other organs and tissues are Inaccessible.


It showed that the permeability of erythrocytic membrane increases upon exposure to polyene antibiotic such as amphotericin B. Resaled, these methods induce irreversible destructive changes in the cell membrane and hence are not very popular. Red cell recovery may be calculated on the basis of the differences in the hematocrit and the volume of the suspension of erythrocytes before and after loading.

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Packed loaded cells are deproteinized with acetonitrile after centrifugation at rpm far a fixed time interval. The haematocrit is defined as the percentage of whole blood made up of erythrocytes. Hypotonic preswell technique This method based on the principle of first swelling the erythrocytes without lysis by placing them in slightly hypotonic solution.

In the 19 th century, Hope Seyler identified hemoglobin and its crucial role in oxygen delivery to various parts of the body.


The content of hemoglobin of the erythrocytes rsealed be diminished by the alterations in the rseealed of the membrane of the red cells during the encapsulation procedure. This method has been used for loading enzymes such as galactosidase, glucoserebrosidase, asparginase, inositol hexaphosphatase, as well as drugs such as gentamicinadriamycin, pentamidine and furamycin, interlukin-2, desferroxamine and human recombinant erythropoietin This reduces the circulation half life of the loaded cells.


Erythrocytes as Carriers for L-Asparaginase: Diffusion through the membrane of the cell and 3. Erythrocytes as a carrier for prednisolone – in vitro and in vivo evaluation. Int J Pharm; Erythrocytes based delivery system with their ability to provide controllled and site specific drug delivery will revolutionize disease management.

Enzyme Loading of Erythrocytes.

Proc Natl Acad Sci ; Go to Application Have a question? Harper Collins College Publishers. The goal is to minimize the loss during the encapsulation procedure to maximize cell recovery Thus, the major fraction of oxygen bound to hemoglobin is recirculated with venous blood to the lungs.

The solution tonicity is then restored by adding a hypertonic buffer. Indian Drugs ; Applications of released erythrocytes. Several methods are based on the principle that semipermeable dialysis membrane maximizes the intracellular and extracellular volume ratio for macromolecules during lysis and resealing.

Eur J Clin Inves; 16 1: