31 Jul In book: Shafer’s Textbook of Oral Pathology: 7th Edition, Edition: 7 th Edition, Chapter: Routine Histotechniques, Staining and Notes on. 5 Mar Histology refers to the study of microscopic structures in biological material and its ways where individual components are both structurally and. Histology Lab, Biology Spring Dr. Ed Devlin. Webpage for Course: Lab Topic.

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In order histogechniques accomplish this it may be necessary to reduce the diameter of the field diaphragm in order to see its edges clearly when you examine a specimen as in step 4. The glass slides are then placed in a warm oven for about 15 minutes to help the section adhere to the slide. Many of them are based on limolene, a volatile oil found in citrus peels. This can be confirmed by polarized light microscopy, because this pigment will polarize a bright white and the slide will look like many stars in the sky.

Motor end organs w.


You may wish to try this as an experiment. Following the uistotechniques the specimens must be washed to remove the fixative this is usually done with water or ethanol. One way to partially solve the problem is to change the fixative at intervals to avoid exhaustion of the fixative.

Methyl methacrylate is very hard and therefore good for embedding undecalcified bone. However, the mercury deposits must be removed dezenkerized before staining or black deposits will result in the sections. If you do not have one, don’t worry about it. Place a slide containing a stained section on the stage of the microscope and examine it using the 10X objective.


Introductory Histotechniques – Discipline of Pathology – The University of Sydney

Uterus hiistotechniques progravid phase sec. The next step in the process involves the removal of water from the specimen. This is a good opportunity to be able to examine a typical cell at higher magnification and resolution using electron micrographs photographs of cells taken on a transmission electron microscope.

Sections cling to block instead of knife.

Histo Techniques

The following factors are important in how the image of microscopic specimens will appear: Because class size is limited to eight people, there is ample opportunity to tailor certain parts to individual needs. Laboratory accidents must be documented and investigated with incident reports and industrial accident reports. Staining a frozen section.

The numerical aperture of the objective lens. Do not soak nervous system tissues at any time and lymph nodes and fatty tissue histotechniiques briefly.

They are highly volatile and if the tissue is exposed to the air the clearing agents will evaporate and leave air pockets in the tissue. It is suitable for beginners and those who would like to refresh their knowledge and practical skills.

It has an unknown mechanism of action. Different colored inks can be used to identify different areas if needed. Ovary corpus luteum of pregnancy sec.


Lymphocytes can be identified by their large nucleus with masses of chromatin material. Hematoxylin will not directly stain tissues, but needs a “mordant” or link to the tissues. These fixatives penetrate relatively poorly and cause some tissue hardness, but histotechniquse fast and give excellent nuclear detail. Be Careful With The Instrument.

Tissue specimens received in the surgical pathology laboratory have a request form that lists the patient information and history along with a description of the site of origin. The histotechniquea tissue processor is one of the commonest and most reliable a mechanical processor with an electric motor that drives gears and camsthough no longer made.

Switch to the 40X objective. In this exercise we will examine how the refractive index of three types of mounting media effects the histotrchniques of the specimen we hiwtotechniques.

Therefore, formaldehyde is good for immunohistochemical techniques. Bouin’s solution is sometimes recommended for fixation of testis, GI tract, and endocrine tissue. After the field diaphragm is in focus, with a given objective lens 10X, 40X, Xopen the diaphragm until the light just fills the field of view as seen through the eyepieces.