API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
|Published (Last):||10 January 2006|
|PDF File Size:||8.42 Mb|
|ePub File Size:||11.1 Mb|
|Price:||Free* [*Free Regsitration Required]|
Each strip consists of 12 different biochemical substrates. H 2 200e is produced from thiosulphate. Used materials should be autoclaved, incinerated, or immersed in germicide before disposal.
API PANEL READING
This causes a pH rise as indicated by bromothymol blue. This code is entered into the computer package. Using the results obtained, from each group of three reactions a 9 nine digit code number is produced. Microbact Biochemical Identification Kits. Gaelrie the results obtained, the indices of the positive reactions are circled. All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites aapi give a positive reaction.
Well 8 for 12B and 20 for 24E is not overlayed with oil for oxidase- positive, miscellaneous Gram-negative bacilli. After the addition of Nitrate reagents A and B: Bromothymol blue indicator changes from blue to yellow when the carbohydrate is fermented.
The sum of these indices in each group of three reactions forms the code number. General Aspects and Perspectives. An organism unable to utilize this substrate results in the accumulation of succinic acid and the organism cannot grow. Once the foil pouch has been opened, unused strips must be placed back in the foil pouch, and the foil pouch taped closed.
Sodium malonate is the sole carbon source and this inhibits the conversion of succinic acid to fumaric acid. The results of tests for oxidase, nitrate reduction and motility are included as part of the reaction pattern.
Set up proceedure Isolation An hour pure culture of the organism to be identified must be obtained.
The pH shift indicated by bromothymol blue caused by formation of the specific gslerie putrescine is greater than that caused by lysine decarboxylation. Materials Required but not provided The following materials may be required but are not provided: Citrate utilization citrate is the only source of carbon.
Reactions obtained using the Microbact System may differ from published results using other substrate formulations. The arginine reaction well 12 for 12B and well 24 of 24E is interpreted differently at 24 hours and 48 hours incubation.
Materials Required but not provided The following materials may be required but are not provided:. Evaluate galerle 2 minutes of the addition of the reagent. The results should be interpreted as follows:.
Solid gelatin particles which may drift across the well after rehydration should be considered as a negative reaction. Organism identification is based on pH change and substrate utilisations as established by published reference methodologies 4,6,8,9.
H 2 S reacts with ferric salts in the medium to form a black precipitate. The 12B strip can be used in conjunction with the 12A strip for the identification of oxidase-positive, nitrate-negative, and glucose-nonfermenters MGNB as well as the Enterobacteriaceae. Well 8 Indole production – add 2 drops of Indole Kovacs reagent. Remove the strips or tray from the incubator, peel back the sealing tape. Motility and DNase tests are recommended for further speciation of this group.
API for Microorganism Identification | bioMérieux
galeroe Using a sterile Pasteur pipette, transfer one drop of the peptone water culture into the appropriate volume see Procedure Chart of sterile saline solution 0.
Argine dihydrolase converts arginine into ornithine, ammonia and carbon dioxide. Strips stored in this manner must be used within 7 days. All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred.
Tryptophan deaminase forms indolepyruvic acid from tryptophan which produces a brown colour in the presence of ferric ions. The 24E solid microplate format contains the 24 substrates contained in the combination of both the 12A and 12B strips.
API®/ID32 | bioMérieux Industry website
Kit presentation Each kit contains the following: Yellow-green is indicative of a negative result. The reactions are evaluated as positive or negative by comparing them with the colour chart. In addition, gxlerie and nitrate test should be performed for miscellaneous Gram-negative bacilli.
The lysine and ornithine decarboxylase reactions should be carefully interpreted. Qualified Personnel should collect specimens according to standards routinely required for specimen handling Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2. Inoculation The wells of individual substrate sets can be exposed by cutting the end tag of the sealing strip and slowly peeling it back.
Twelve substrates provide insufficient data to speciate within this group as a single aberrant reaction may result in an incorrect identification.