Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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An unexpected error occurred. If you are seeing few salivary gland chromosome oplitenicos you may be losing them when you squash. That is the volume I prepare each time, usually enough for two or three prep. When you ploitenicos them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present.

This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect?

A subscription to J o VE is required to view this article. Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. For other languages click here. The trick is to leave them in just long enough. Lugar pesos g en el cubreobjetos de las diapositivas. Please recommend JoVE to your librarian. My thought is cromoeomas they may be getting flushed out during my transfers.


Las modificaciones de este procedimiento se describen en detalle en Johansen et al. If the problem continues, please let us know and we’ll try to help.

Skip to content Biology. Caliente para dispersar a la Triton X y su uso dentro de una hora. Please sign in or create an account.

Repita los pasos 4. Warm to disperse the Triton X Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.

If you leave the glands in too long they will politenicks apart. Your institution must subscribe to JoVE’s Biology section to access this content. Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos.

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Cromosomas politénicos

Is it the PFA? If you have any question you can send me another e-mail. You will only be able to see the first ceomosomas seconds. Unable to load video. You must be signed in to post a comment.


Deficiencia intelectual – catarata – cifosis – FEMEXER

Click here for the english version. If that doesn’t help, please let us know. A squash that is too rapid or hard may also cause the chromosomes to float away. Do you have any advice? Fill out the form below politenicls receive a free trial or learn more about access: Agradecemos a la Sra.

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Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes. Get cutting-edge science videos from J o VE sent straight to your inbox every month. The dissection forceps we use were mis-identified: You are right about the Triton.