BIOASSAY OF RABIES VACCINE PDF

It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. 7 Aug BIOLOGICAL ASSAY OF RABIES VACCINE: PRINCIPLE: The potency of rabies vaccine is determined by comparing a lethal intracerebral dose. 30 Oct BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.

Author: Mikale Vikora
Country: Bulgaria
Language: English (Spanish)
Genre: Art
Published (Last): 17 August 2013
Pages: 90
PDF File Size: 9.99 Mb
ePub File Size: 14.64 Mb
ISBN: 592-5-52724-274-9
Downloads: 35458
Price: Free* [*Free Regsitration Required]
Uploader: Kigataxe

LP means limes paralyticum This is the smallest quantity of the toxin when mixed with 0. Record the contraction produces by the addition of two dose of std. Cats are more likely to be aggressive than dogs Animals exhibit change in instinctive behavior: Stay min protected from the light.

Sequential Steps of Rabies Transmission. On the other hand another vaccine, derived from the LEP bjoassay, was considerably more potent in antigenicity assays than in immunogenicity assays. You do not have the permission to view this presentation.

Standard preparation is dried serum or Other preparationthe potency of which has been determined in relation to international standard. A collaborative study was carried out to establish a replacement for the International Standard for Rabies Vaccine, the stocks of which are bioaxsay. Mice, 10gg -animal same sex. The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of rabies virus with the dose of standard preparation of rabies antiserum necessary to give same protection.

Suspended cells in 10ml of sterile suspension medium.

Vaccines |authorSTREAM

Presentation Description No description available. Oxytocin stimulate the contraction of the uterine smooth muscle and the memory gland. Tetanus anti-toxin is a preparation containing antitoxic globulins that have the power of specifically neutralizing the toxin formed by clostridium tetani.

  FOCAL 165-K2P PDF

Severity of exposure Location of the bite The biting animal Bites on head and neck have shorter incubation time as short as 15 days because of rich peripheral nerve supply. The results of this study indicate that the NIH protection test should continue to form the primary basis for potency assay of rabies vaccine as glycoprotein content does not appear to correlate with immunogenic potency for different types of vaccine.

Rabies VACCINE – JSK NAGARAJAN |authorSTREAM

Protected from the light. The proposed fifth International Standard PISRAV which was derived from the same virus strain as the present international standard preparation, the Pitman Moore PM strain, was found to be approximately twice as potent relative to the International Standard in immunogenicity assays as in antigenicity assays.

bioaszay The prepared plates or dishes must be stored such that no significant growth of the test microorganism occurs before use, and the surface of the agar layer vacclne dry at the time of use. National Center for Biotechnology InformationU. It is obtained by fractionation from horse serum or other mammals that have been immunized against tetanus toxin.

The volume of solution added to each cylinder or cavity must be uniform and sufficient to fill the holes. The vaccine is prepared immediately before use by reconstitution from the dried vaccine with a suitable sterile liquid.

These two groups vacfine used for titration of LD50 of challenge suspension. Transmission Almost all transmission is by bite 50 times greater risk than a scratch One human case may have been acquired in a laboratory transmitted by aerosol In wild animals: Test dose and std dose contains 0.

The toxin should be selected for assay. P Exposure the gluteus primus muscle thigh and rrabies politeal artery and crural vein.

  GARUDA KAVACHAM PDF

Animal Behavior Classic Picture of rabid, mangy dog foaming at the mouth…not often seen, signs frequently more subtle. Arbies all the response and calculate the result of the assay by standard statistical method. Didn’t get the message? Discard any values falling beyond the range.

If it is derived from animal species. Three rabies vaccines for human use derived from different rabies virus strains and prepared on different cell culture substrates were compared with the International Standard for Rabies Vaccine using in vivo and in vitro assay methods in a collaborative study involving 14 participants.

Any suitable strain rabies virus of known potency, such as the CVS strain may rabues used. Generate a file for use with external citation management software. It is a neurosecretary product mainly synthesize in the cell bodies of paraventracular nuclear of the hypothalamus.

P return to normal Dilute the preparation with biooassay solution so as to get same response as standard The ratio between standard and test should be equal If the animal rapidly becomes insensitive to the repeated injection the solution another must be used.

Please review our privacy policy.

We use cookies to help provide and enhance our service and tailor content and ads. The presentation is successfully added In Your Favorites. Upload from Desktop Single File Upload.

Consciousness initially spared Two forms can overlap or progress from one to the other Coma after one week of neuro symptoms with death a few days after.